Faculty of Medicine Foča, University of East Sarajevo, Lukavica, Bosnia and Herzegovina
Oncology Institute of Vojvodina, Novi Sad, Serbia
Institute for Pulmonary Diseases of Vojvodina, Kamenica, Serbia
University clinical center of Republika Srpska, Banja Luka, Bosnia and Herzegovina
Faculty of Medicine Foča, University of East Sarajevo, Lukavica, Bosnia and Herzegovina
Faculty of Medicine Foča, University of East Sarajevo, Lukavica, Bosnia and Herzegovina
Faculty of Medicine Foča, University of East Sarajevo, Lukavica, Bosnia and Herzegovina
Faculty of Medicine Foča, University of East Sarajevo, Lukavica, Bosnia and Herzegovina
Faculty of Medicine Foča, University of East Sarajevo, Lukavica, Bosnia and Herzegovina
Introduction. Extramural venous invasion (EMVI) is a significant predictive factor of the prognosis for patients with colorectal carcinoma and it is directly connected with the relapse of a disease, especially with the appearance of distant metastases. Methods. The research comprises 90 patients with colorectal cancer. Representative samples of tumor tissues obtained by surgical resection are fixed in 4% formalin and embedded into paraffin blocks. Semi-series incisions of 4μm thickness were stained by HE method and Van Gieson’s method. Results. Out of 90 analyzed patients, 21 (23,33%) were with EMVI, and 69 (76.67%) without EMVI on the histological preparations stained by HE method. EMVI was found in 7 more tumors on the preparations stained by Van Gieson’s method. Sensitivity of the method determining EMVI on the histological preparations stained by Van Gieson’s method was better for 25% (7/28) than on the preparations stained by HE method. EMVI was not found in patients at A and B1 stage of disease according to Astler- Coller classification, but out of 77 patients which were at B2, C1 and C2 stage EMVI was found in 28 (36,36%) patients, p≤0,01. EMVI was found in 18/77 (23,38%) patients who had a tumor of low histological gradus, p≤0,01 and in 10/13 (76,92%) patients who had a tumor of high histological gradus, p≤0,01. In patients with neural invasion, EMVI was found in 18/22 (81,82%), whereas in patients without neural invasion EMVI was found in 10/68 (14,71%), p≤0,01. In patients with weak intensity of peritumoral lymphocytic reaction EMVI was found in 20/41 (48,78%) whereas in patients with moderate and noticeable level it was found in 8/49 (16,33%), p≤0,01. There was a significant correlation between extramural venous invasion and other parameters of unfavorable prognosis. Conclusion. EMVI is an important indicator in administration of postoperative adjuvant therapy in patients with colorectal carcinoma. A special histochemical method of elastic fiber stain should be applied in everyday practice when EMVI is not found on HE stained preparations in patients with CRC.
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